Figure 5.
Schematic of the CD protocol used to prepare asymmetric vesicles. Donor vesicles with desired outer leaflet lipid composition are mixed with CD (represented by the trapezoids) and introduced to acceptor vesicles with the desired lipid composition in their inner bilayer leaflets. After incubating the Cd-lipid mixture, vesicles exchange their outer bilayer leaflets, resulting in asymmetric acceptor vesicles with the desired lipid compositions in both their inner and outer leaflets. Adapted from [50].