IC50 concentrations (μM) of lead TMZ analogues against U87-MG, 1321-N1, GOS-3 and the normal SVGp12 cell linea.
| Analogue | IC50b (μM) | |||
|---|---|---|---|---|
| U87-MG | 1321-N1 | GOS-3 | SVGp12 | |
| TMZ | 621 ± 66 | 783 ± 40 | 696 ± 16 | 239 ± 6 |
| TMZ acid | 662 ± 42 | 596 ± 26 | 504 ± 30 | 316 ± 2 |
| 3b | 409 ± 45 | 269 ± 20 | 269 ± 6 | 182 ± 11 |
| 3h | 134 ± 7 | 183 ± 1 | 183 ± 5 | 44 ± 2 |
| 3i | 141 ± 3 | 104 ± 4 | 91 ± 1 | 39 ± 2 |
| 3j | 96 ± 3 | 118 ± 11 | 54 ± 4 | 33 ± 1 |
| 3k | 137 ± 7 | 103 ± 7 | 151 ± 4 | 36 ± 3 |
| 3l | 330 ± 47 | 511 ± 10 | 691 ± 35 | 238 ± 6 |
| 3n | 376 ± 62 | 238 ± 8 | 314 ± 38 | 215 ± 26 |
| 4k | 138 ± 28 | >200 | >200 | 79 ± 6 |
a
All the data were the average values of three independent assays, IC50 ± SD (n = 3).
b
Compound concentration that reduces cell viability by 50% compared to untreated cells.