Verification of the recovery rates in various concentrations (in acidic soil, pH 5.52)a.
| Compd | HPLC analysis condition | Extraction solvent (v/v) | Additive concentration (mg kg−1) | Average recovery ratio (%) | Coefficient of variation R2 (%) | ||
|---|---|---|---|---|---|---|---|
| Wavelength | Flow rate | Mobile phase (v/v) | |||||
| M01 | 225 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 53/47 | CH3COCH3 : CH2Cl2 : phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 79.64 | 3.98 |
| 2 | 82.65 | 1.55 | |||||
| 0.5 | 87.11 | 1.97 | |||||
| M02 | 225 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 53/47 | CH3COCH3 : CH2Cl2 : phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 83.39 | 1.47 |
| 2 | 84.58 | 2.29 | |||||
| 0.5 | 95.83 | 1.10 | |||||
| M03 | 225 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 60/40 | CH3COCH3 : CH2Cl2 : phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 81.13 | 2.57 |
| 2 | 76.66 | 2.37 | |||||
| 0.5 | 75.89 | 2.47 | |||||
| M04 | 225 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 65/35 | CH3COCH3 : CH2Cl2 : Phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 81.80 | 3.25 |
| 2 | 79.78 | 1.25 | |||||
| 0.5 | 78.59 | 0.90 | |||||
| M05 | 225 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 63/43 | CH3COCH3 : CH2Cl2 : phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 81.00 | 1.56 |
| 2 | 78.41 | 3.85 | |||||
| 0.5 | 73.62 | 2.93 | |||||
| N01 | 235 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 70/30 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 88.92 | 2.23 |
| 2 | 85.45 | 2.87 | |||||
| 0.5 | 75.15 | 1.66 | |||||
| N02 | 230 nm | 0.9 mL min−1 | CH3OH : H2O (pH 3.0) = 75/25 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 79.52 | 0.84 |
| 2 | 73.57 | 2.41 | |||||
| 0.5 | 72.58 | 0.49 | |||||
| N03 | 225 nm | 0.7 mL min−1 | CH3OH : H2O (pH 3.0) = 72/28 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 88.15 | 3.09 |
| 2 | 85.01 | 0.80 | |||||
| 0.5 | 80.00 | 0.87 | |||||
| N04 | 230 nm | 0.7 mL min−1 | CH3OH : H2O (pH 3.0) = 68/32 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 84.53 | 0.40 |
| 2 | 73.51 | 2.89 | |||||
| 0.5 | 83.89 | 1.86 | |||||
| N05 | 230 nm | 1.0 mL min−1 | CH3OH : H2O (pH 3.0) = 75/25 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 95.28 | 1.15 |
| 2 | 92.11 | 2.38 | |||||
| 0.5 | 85.50 | 2.92 | |||||
| N06 | 230 nm | 1.0 mL min−1 | CH3OH : H2O (pH 3.0) = 75/25 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 36/12/12/12 | 5 | 75.33 | 3.30 |
| 2 | 76.97 | 1.92 | |||||
| 0.5 | 75.13 | 1.10 | |||||
| N07 | 225 nm | 0.9 mL min−1 | CH3OH : H2O (pH 3.0) = 78/22 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 1.5) = 36/15/15/12 | 5 | 77.05 | 2.48 |
| 2 | 79.71 | 2.04 | |||||
| 0.5 | 79.79 | 0.61 | |||||
| N08 | 230 nm | 0.9 mL min−1 | CH3OH : H2O (pH 3.0) = 75/25 | CH3COCH3 : THF : CH2Cl2 : H3PO4 aqueous solution (pH 2.0) = 30/10/10/10 | 5 | 74.10 | 2.96 |
| 2 | 78.17 | 2.25 | |||||
| 0.5 | 74.32 | 2.69 | |||||
| N09 | 225 nm | 0.9 mL min−1 | CH3OH : H2O (pH 3.0) = 76/24 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 80.53 | 7.16 |
| 2 | 73.28 | 2.38 | |||||
| 0.5 | 74.06 | 0.37 | |||||
| N10 | 230 nm | 1.0 mL min−1 | CH3OH : H2O (pH 3.0) = 78/22 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 1.5) = 36/12/12/5 | 5 | 75.01 | 2.88 |
| 2 | 74.30 | 2.90 | |||||
| 0.5 | 77.05 | 2.40 | |||||
| N11 | 230 nm | 1.0 mL min−1 | CH3OH : H2O (pH 3.0) = 80/20 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 1.5) = 36/12/12/5 | 5 | 72.75 | 2.14 |
| 2 | 77.47 | 1.01 | |||||
| 0.5 | 72.61 | 2.43 | |||||
| N12 | 230 nm | 0.9 mL min−1 | CH3OH : H2O (pH 3.0) = 75/25 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 77.35 | 1.24 |
| 2 | 84.32 | 2.94 | |||||
| 0.5 | 86.93 | 2.10 | |||||
| N13 | 230 nm | 1.0 mL min−1 | CH3OH : H2O (pH 3.0) = 80/20 | CH3COCH3 : THF : CH2Cl2 : phosphoric acid solution (pH 2.0) = 30/10/10/10 | 5 | 75.17 | 2.22 |
| 2 | 72.08 | 0.82 | |||||
| 0.5 | 77.26 | 2.35 | |||||
| Chlorsulfuron | 235 nm | 0.8 mL min−1 | CH3OH : H2O (pH 3.0) = 60/40 | CH3COCH3 : CH2Cl2 : phosphoric acid solution (pH 2.0) = 40/5/5 | 5 | 79.41 | 2.32 |
| 2 | 79.34 | 2.26 | |||||
| 0.5 | 83.54 | 3.46 | |||||
H2O (pH 3.0) was acidified with H3PO4.