List of metabolites in leaves of cucumber seedlings whose relative fold changes were significantly down-accumulated (≤0.5) after Cd treatment and up-accumulated (≥1.5) or unchanged (0.5< and <1.5) after Cd + Se-treatment.
| Metabolite name | Similarity | R.T.a | Mass | VIPb | P valuec | Fold changed | Pathway | |
|---|---|---|---|---|---|---|---|---|
| Cd vs. control | Cd + Se vs. Cd | |||||||
| Pyruvic acid | 927 | 7.2052 | 174 | 1.87353 | 0.01560 | 0.35 | 1.98 | Glycolysis |
| Galactose 1 | 939 | 17.7571 | 319 | 2.00540 | 0.00810 | — | 2.40 | Glycolysis |
| Lactose 1 | 801 | 24.6819 | 204 | 1.66282 | 0.03241 | — | 4.99 | Glycolysis |
| Lactic acid | 968 | 7.3206 | 117 | 1.90526 | 0.01573 | — | 0.72 | TCA cycle |
| Oxalic acid | 704 | 8.3484 | 147 | 1.96350 | 0.00734 | — | 1.22 | TCA cycle |
| 3-Hydroxypropionic acid 1 | 757 | 8.5775 | 177 | 2.43025 | 0.00011 | — | 4.59 | TCA cycle |
| 2,3-Dimethylsuccinic acid | 395 | 11.4907 | 221 | 1.82082 | 0.02495 | — | 1.21 | TCA cycle |
| Glutaric acid | 757 | 12.2118 | 55 | 2.52714 | 0.00002 | — | 7.06 | TCA cycle |
| Citramalic acid | 921 | 13.0089 | 247 | 1.82753 | 0.01967 | — | 1.84 | TCA cycle |
| Glycero | 904 | 10.3819 | 117 | 1.89634 | 0.01340 | 0.44 | 1.23 | Sugars and polyols |
| 3-Methylamino-1,2-propanediol 2 | 464 | 11.7196 | 117 | 1.73584 | 0.01805 | — | 1.19 | Sugars and polyols |
| d-Glycerol 1-phosphate | 871 | 16.4002 | 299 | 1.65905 | 0.04984 | — | 1.75 | Sugars and polyols |
| Glucoheptonic acid 3 | 673 | 20.1423 | 319 | 2.14536 | 0.00387 | 0.46 | 2.59 | Sugars and polyols |
| Phytol | 972 | 20.5121 | 143 | 2.17178 | 0.00227 | 0.50 | 1.91 | Sugars and polyols |
| Melibiose 2 | 840 | 26.0556 | 361 | 1.95062 | 0.01075 | — | 4.56 | Sugars and polyols |
| Alanine 1 | 957 | 7.9520 | 116 | 2.01987 | 0.00487 | 0.41 | 1.51 | Amino acid |
| Glycine 2 | 947 | 10.8449 | 174 | 2.41138 | 0.00015 | 0.29 | 4.47 | Amino acid |
| Aminomalonic acid | 676 | 12.9948 | 218 | 2.32434 | 0.00287 | 0.13 | 5.91 | Amino acid |
| Palmitoleic acid | 877 | 19.1547 | 55 | 1.75218 | 0.02081 | — | 1.43 | Lipids |
| Linoleic acid | 923 | 20.8606 | 337 | 1.83842 | 0.01769 | — | 1.90 | Lipids |
| 4-Hydroxybutyrate | 819 | 9.9014 | 233 | 2.47526 | 0.00004 | — | 4.45 | Others |
| Nornicotine | 431 | 13.9813 | 142 | 1.93520 | 0.00797 | — | 2.30 | Others |
| Phthalic acid | 504 | 15.8280 | 304 | 1.77004 | 0.04181 | 0.28 | 2.94 | Others |
| N-Acetyl-beta-d-mannosamine 4 | 771 | 19.8795 | 319 | 1.95543 | 0.00945 | 0.32 | 2.82 | Others |
a
R.T. represents the retention time.
b
VIP = variable importance projection, metabolites (VIP > 1) are listed in the table.
c
P values were calculated according to Student's T-test.
d
Fold change is represented as the ratio of the peak intensities for the corresponding treatments (n = 6), —: represented, not detected.