Table 1.

Purity and yield of SVs isolated by rho1D4-IP

Fraction	Volume, ul	[Protein], ng/ul	[Synaptophysin], normalized	Enrichment factor	Percentage of input synaptophysin	Preparation time, minutes
Input (supernatant from one-half mouse brain)	1900	2500 ± 300	1	1	100	30
rho1D4 bead-bound material	∼60	95 ± 11	7.7 ± 0.7	180 ± 30	∼20%	60
1D4 peptide eluate	∼60	25 ± 7.5	3.3 ± 0.6	330 ± 120	∼10%	90

Quantification of input protein was achieved by BCA assay, and quantification of SV yields was achieved as described in Materials and Methods. Data are shown as mean ± SEM for three immunoprecipitations using two mouse brains and three batches of rho1D4 beads. In contrast, SVs prepared using classical methods usually achieve synaptophysin enrichment factors of 20–30 when adjusted for the type of input fraction used here (Ahmed et al., 2013), whereas the SV-tag approach achieves an enrichment factor of 10–15 for synaptobrevin (Chantranupong et al., 2020).