Skip to main content
. 2020 May 19;10(32):18970–18977. doi: 10.1039/d0ra02104k

Fig. 3. Confocal fluorescence micrographs obtained using the ghrelin–formazanate probe 5. Red images (from 5): excitation at 559 nm and emission collected between 598–698 nm. Blue images (DAPI): excitation at 405 nm and emission collected between 425–475 nm. (a) Imaging 5 with OVCAR-8 cells transfected with GHSR-1a (40× objective). (b) Imaging DAPI with OVCAR-8 cells transfected with GHSR-1a and overlay of image (a). (c) Imaging 5 with OVCAR-8 cells transfected with GHSR-1a using 60× objective. (d) Imaging DAPI with OVCAR-8 cells transfected with GHSR-1a and overlay of image (c), using 60× objective. (e) Imaging 5 with parental OVCAR-8 cells (40× objective). (f) Imaging DAPI with parental OVCAR-8 cells and overlay of image (e). (g) Imaging 5 OVCAR-8 cells transfected with GHSR-1a and blocked with antagonist 8 (40× objective). (h) Imaging DAPI with OVCAR-8 cells transfected with GHSR-1a and blocked with antagonist 8 and overlay of image (g). Scale bar 20 μm except (c) and (d) 10 μm.

Fig. 3