Fig. 4. Four sequence blocks in FCHO linkers and the effects of their deletion on AP2 binding.

(A) Human FCHO2 (FC2 top) and FCHO1 (FC1 bottom) interdomain linker sequences aligned with identities highlighted in black and conservation in gray. Consensus CK2 phosphorylation sites are shown in dark pink and hierarchical CK2 sites in light pink. The conserved sequences blocks N1, N2, N3, and C assessed on the basis of conservations between sequences from across species and in the case of FCHO2, also on the basis of structure, are colored yellow, orange, red, and claret, respectively (coloring maintained throughout all subsequent figures). (B) Summary of the binding of the deletion constructs indicated on the unstructured FCHO2 and FCHO1 linkers to recombinant AP2 core and AP2 from brain cytosol as indicated. Gels shown in fig. S3.