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. 2022 Apr 29;8(17):eabn0044. doi: 10.1126/sciadv.abn0044

Fig. 6. WT and E83Q fibrils preferentially seed the aggregation of monomers with the same sequence.

Fig. 6.

(A) ThT-based aggregation kinetics of cross-species aggregation of human WT (hWT) monomers with hE83Q monomers at varying ratios. (B) Bar diagram showing the lag phase (hours) extracted from the aggregation kinetics [from (A)]. (C) Negatively stained EM images of the fibril samples at the end time point of the ThT kinetics [from (A)]. Scale bars, 100 nm. (D and E) ThT-based aggregation kinetics of seeding of 10 μM concentration of WT monomers (D) or E83Q monomers (E) with 2% of WT PFFs or E83Q PFFs. (F and G) EM analysis of end time point [from (D) and (E)] samples of WT monomers alone and seeded with 2% E83Q PFFs (F) and E83Q monomers alone and seeded with WT PFFs (G) and their fibril length distribution as the dot plot graphs, respectively. Scale bars, 100 nm.