Table 1. qRT-PCR primer sequences.
Gene | Forward | Reverse |
---|---|---|
LDH | 5′-GCAGCAGGGTTTCTATGGAG-3′ | 5′-TGGAGACAGTGGGATTGTCA-3′ |
NRF-2 | 5′-GCACTCTGTGGAGTCTTCCATTTA-3′ | 5′-GAAGAATGTGTTGGCTGTGCTTTA-3′ |
COX-IV | 5′-GAACAAGGGCACCAATGAGT-3′ | 5′-GTTGACCTTCATGTCCAGCA-3′ |
PGC-1α | 5′-AAACTGCAGATTTGATGGACC-3′ | 5′-TTTCCCTCTTCAGCATAGTTC-3′ |
Cyclophilin | 5′-TGGCAAGCATGTGGTCTTTGGGAAG-3′ | 5′-GGTGATCTTCTTGCTGGTCTTGCCATTC-3′ |
LDH: lactate dehydrogenase; NRF2: nuclear factor erythroid-2 related factor 2; COX-IV: cytochrome oxidase IV; PGC-1α: peroxisome proliferator-activated receptor gamma coativator-1 alpha.