Skip to main content
. Author manuscript; available in PMC: 2022 Oct 12.
Published in final edited form as: Nat Cell Biol. 2022 Apr 12;24(4):434–447. doi: 10.1038/s41556-022-00888-x

Extended Data Fig. 7. Apyrase treatment partially blocks EMT.

Extended Data Fig. 7

A) Timeline of apyrase-treated conditioned media (CM) experiment. WT hiPSC were initially treated with CHIR + Q-VD-OPH. At the same time, WT hiPSCs were treated with CHIR only. 24hrs later, Q-VD-OPH was washed out and condition media from the CHIR-only treatment was added onto the Q-VD-OPH pre-treated cells, in the presence or absence of 2U/mL of Apyrase.

B) Representative Max IPs pictures of WT cells fixed after adding apyrase-treated CM as presented in (A). Cells were stained for ZO-1, Slug and nuclei. Scale bar = 50 μm. Magnified area (yellow dotted square) is shown for the ZO-1 channel. Scale bar = 10 μm.