Comparison of key properties between immobilised and free GOase M₁.

	Free	Immobilised
Half-lifea	<24 h	>3 weeks
Thermal activityb	0.39	1.52
Thermal stabilityc	0.38	1.25
Solvent toleranced	No	Yes

^aHalf-life is the point at which the enzyme retains 50% of the original activity.

^bActivity measured at 60 °C and is relative to free enzyme activity at 25 °C.

^cStability is the recovered relative activity of the enzyme at 25 °C after incubation at 60 °C for 17 h.

^dSolvent tolerance determined as the ability to turn over substrate in neat organic solvent.