Appendix 1—figure 8. GC clusters in origins and alternate structures without origins.

(a) A visualization of the distinct GC clusters within all eight origins of replication. A 10 bp sliding window is moved along the origin sequences. GC clusters are the 3–4 distinct peaks in each of the origin sequences, all above 0.6 GC content in the sliding window which are consistent with the locations described in de Zamaroczy and Bernardi, 1986. (b) A visualization of the GC clusters within observed alternate structures without canonical origins of replication. These clusters at similar GC content (0.6 and above) may act as surrogate replication origin sites.