TABLE 2.
Comparison of antimicrobial activity and adhesive activities among different Lactobacillus strains
Lactobacillusa | Log10 difference in bacterial countb | Adhesive propertyc
|
||||
---|---|---|---|---|---|---|
Fibronectin binding (%)
|
Platelet aggregation (lag phase, min) | Polystyrene adhesion (%) | Salivary aggregation (%) | |||
pH 7.3 | pH 5.4 | |||||
L. casei strain Shirota | 2.2∗ | 0 | 1 | 0 (25<) | −5 | 0 |
L. fermentum ATCC 14931T | 0.2 | 6 | 77 | 0 (25<) | 0 | −5 |
L. jensenii ATCC 25258T | −0.4 | 1 | 8 | 0 (25<) | 5 | 75 |
L. plantarum ATCC 14917T | −0.6 | 37 | 83 | 103 (13) | 1 | −8 |
L. reuteri JCM 1112T | 0.5 | 8 | 48 | 0 (25<) | 33 | 27 |
Each strain used was grown in MRS broth for 24 h at 37°C.
Viable cells of each strain at an inoculum (L. casei Shirota, 1.8 × 108 CFU; L. fermentum, 1.0 × 108 CFU; L. jensenii, 1.0 × 108 CFU; L. plantarum, 2.2 × 108 CFU; L. reuteri, 1.9 × 108 CFU) were instilled intravesically on day −1, and the mice were infected with E. coli strain HU-1 (2 × 106 CFU) intravesically on day 0. Mice were dissected 24 h after the infection. The antimicrobial activity of each Lactobacillus strain is expressed as the log10 difference in the bacterial count, calculated as the log 10 number of E. coli bacteria in the bladders of mice pretreated with saline minus the log10 number of E. coli bacteria in the bladders of mice pretreated with Lactobacillus strains. Six mice per group were used. ∗, P < 0.05.
Determined as described in Materials and Methods.