Fig. 4. SHP099 release and change in film thickness over time. (a) Normalized cumulative release (percent of total loading) of SHP099 from (CHT/PβCD)₂₅ films with SHP099 loaded “pre-assembly” and “post-assembly” incubated in 1× PBS, pH 7.4 at 37 °C. Inset: Release in first 5 hours. (b) Concentration of SHP099 released from “pre-assembly” and “post-assembly” loaded films over time. Inset: Concentration of SHP099 released at 5 hour time point onward. Red dashed line indicates IC₅₀ of SHP099 for human esophageal and breast adenocarcinoma cell growth inhibition (0.25 μM). (c) Change in film thickness of (CHT/PβCD)₂₅ films loaded with SHP099 (“pre-assembly”) or unloaded over time along with SHP099 release from loaded films. Results are reported as mean ± standard deviation; statistical significance was examined between “pre-assembly” and “post-assembly” loaded films at each time point using Student's t-test (unpaired, two-tailed), n = 3, **p < 0.01, ***p < 0.001. ND: not detectable.