Fig. 3. Binding of compound H4 and H19 with MARK4. (A) Fluorescence emission spectra of MARK4 (5 μM) with increasing concentrations of compound H4 and H19. (B) Stern–Volmer plot obtained from the fluorescence quenching data of MARK4 by compound H4 and H19, respectively. Protein sample was excited at 280 nm and emission for each titration was recorded in the range of 300–400 nm. The value of binding constant/number of binding sites was estimated using Stern–Volmer plot.
