Fig. 4. In vitro cytotoxicity of HeLa cells. MTT assay after treatment for 24 h with (A) G4.5 dendrimer, GH NPs and GHC aggregates; (B) different concentrations of free DOX, (C) apoptosis and necrosis in cell populations were determined using flow cytometry with annexin V/propidium iodide staining (treated with G/DOX, GH/DOX and GHC/DOX for 12 h).