Table 6.
Renal gene expression after 3 wk of DOCA-salt or sham treatment
| Gene | 5L0D Sham (n = 8) |
5L0D DOCA (n = 8) |
2920x Sham (n = 8) |
2920x DOCA (n = 8) |
7913 Sham (n = 8) |
7913 DOCA (n = 8) |
P Value |
||
|---|---|---|---|---|---|---|---|---|---|
| Diet | DOCA | Diet × DOCA | |||||||
| Ren | 1.00 (0.77–1.27) | 0.08 (0.07–0.10)a | 0.72 (0.56–0.93) | 0.28 (0.22–0.35)a,b | 0.94 (0.80–1.11) | 0.17 (0.14–0.20)a | 0.11 | <0.01 | <0.01 |
| Agt | 1.00 (0.85–1.18) | 0.96 (0.88–1.05) | 0.75 (0.66–0.85) | 1.27 (1.18–1.36)a | 0.69 (0.60–0.79) | 1.04 (1.00–1.08) | 0.32 | <0.01 | 0.03 |
| Ace | 1.00 (0.79–1.27) | 0.63 (0.47–0.83) | 0.93 (0.63–1.38) | 0.75 (0.60–0.94) | 0.58 (0.29–1.17) | 0.55 (0.40–0.74) | 0.57 | 0.46 | 0.88 |
| Agtr1a | 1.00 (0.89–1.13) | 0.91 (0.74–1.12) | 1.00 (0.67–1.48) | 0.80 (0.68–0.95) | 0.76 (0.56–1.02) | 0.94 (0.86–1.03) | 0.88 | 0.88 | 0.63 |
| Ncc | 1.00 (0.79–1.27) | 2.60 (1.71–3.98) | 0.39 (0.17–0.89) | 1.23 (0.78–1.95) | 0.70 (0.37–1.35) | 0.82 (0.42–1.59) | 0.39 | 0.16 | 0.71 |
| Nhe3 | 1.00 (0.71–1.42) | 1.06 (0.66–1.70) | 0.40 (0.18–0.88) | 0.58 (0.35–0.95) | 0.46 (0.20–1.03) | 0.42 (0.24–0.75) | 0.35 | 0.83 | 0.93 |
| Nkcc2a | 1.00 (0.81–1.24) | 1.39 (1.07–1.82) | 0.50 (0.33–0.75) | 1.12 (0.92–1.37) | 0.39 (0.20–0.73) | 0.86 (0.65–1.13) | 0.19 | 0.04 | 0.78 |
| Avpr2 | 1.00 (0.81–1.23) | 0.81 (0.70–0.94) | 0.43 (0.35–0.53) | 0.55 (0.50–0.60) | 0.52 (0.44–0.61) | 0.56 (0.49–0.65) | <0.01 | 0.79 | 0.48 |
| Aqp1 | 1.00 (0.83–1.21) | 0.99 (0.74–1.33) | 0.61 (0.41–0.90) | 0.85 (0.70–1.03) | 0.42 (0.21–0.83) | 0.63 (0.47–0.85) | 0.27 | 0.46 | 0.86 |
| Aqp2 | 1.00 (0.81–1.23) | 1.03 (0.88–1.20) | 0.39 (0.30–0.52) | 0.66 (0.55–0.79) | 0.49 (0.37–0.65) | 0.66 (0.52–0.83) | 0.01 | 0.15 | 0.59 |
| Aqp3 | 1.00 (0.70–1.43) | 0.66 (0.46–0.97) | 0.55 (0.28–1.09) | 0.41 (0.28–0.61) | 0.22 (0.08–0.61) | 0.46 (0.27–0.79) | 0.35 | 0.97 | 0.61 |
| Aqp4 | 1.00 (0.83–1.20) | 2.65 (2.35–2.98)a | 1.01 (0.86–1.19) | 1.88 (1.66–2.12)a | 1.18 (1.02–1.37) | 2.42 (2.17–2.70)a | 0.30 | <0.01 | 0.45 |
|
Scnn1a
(ENaC-α) |
1.00 (0.71–1.41) | 2.52 (1.80–3.53) | 0.57 (0.32–1.00) | 1.95 (1.57–2.41) | 0.37 (0.19–0.74) | 1.45 (0.97–2.16) | 0.27 | <0.01 | 0.90 |
|
Scnn1b
(ENaC-β) |
1.00 (0.82–1.22) | 1.53 (1.33–1.75) | 0.68 (0.53–0.86) | 1.23 (1.08–1.39) | 0.53 (0.35–0.80) | 1.17 (1.03–1.34) | 0.15 | <0.01 | 0.72 |
|
Scnn1g
(ENaC-γ) |
1.00 (0.92–1.09) | 1.59 (1.42–1.77) | 0.64 (0.54–0.75) | 1.13 (1.00–1.27) | 0.71 (0.62–0.81) | 0.71 (0.59–0.85) | <0.01 | <0.01 | 0.55 |
|
Atp1a1
(Na-K-ATPase) |
1.00 (0.78–1.28) | 1.62 (1.22–2.17) | 0.62 (0.37–1.03) | 1.50 (1.21–1.86) | 0.37 (0.16–0.82) | 1.06 (0.73–1.54) | 0.33 | 0.04 | 0.83 |
Data presented as means (± SE) fold of 5L0D-sham group and analyzed by two-way ANOVA. aP < 0.05 vs. diet-matched sham, bP < 0.05 vs. 5L0D + DOCA by Tukey’s multiple-comparisons procedure. DOCA, deoxycorticosterone acetate.