Table 4.
Baseline cytokine Consensus Cluster assignments and associated modulations in cigarette smokers and e-cigarette users
| Compartment | Cytokines Within Each Cluster | Nonsmokers, n = 14 vs. Cigarette Smokers, n = 13 Cluster Comparison |
Nonsmokers, n = 14 vs. E-Cigarette User, n = 17 Cluster Comparison |
Cigarette Smokers, n = 13 vs. E-Cigarette Users, n = 17 Cluster Comparison |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| P Value | P adj | Eigencytokine Change in Smokers | P Value | P adj | Eigencytokine Change in E-Cigarette Users | P Value | P adj | Eigencytokine Change in E-Cigarette Users | ||
| Cluster A | ||||||||||
| NLF | IFNg, IL10, IL12p70, IL17D, IL4, TNFa | 7.93E-01 | 7.93E-01 | NA | 7.54E-09 | 2.26E-08 | Increased | 1.67E-08 | 5.01E-08 | Increased |
| NELF | 5.83E-01 | 9.43E-01 | NA | 1.61E-01 | 3.77E-01 | NA | 1.13E-01 | 2.63E-01 | NA | |
| Sputum | 9.43E-01 | 9.43E-01 | NA | 7.99E-01 | 7.99E-01 | NA | 5.63E-01 | 5.63E-01 | NA | |
| Cluster B | ||||||||||
| NLF | Eotaxin, Eotaxin3, IL17B, IL1b, ITAC, MIP1a, TARC | 2.39E-01 | 3.58E-01 | NA | 3.56E-01 | 5.34E-01 | NA | 8.37E-01 | 8.37E-01 | NA |
| NELF | 9.43E-01 | 9.43E-01 | NA | 3.77E-01 | 3.77E-01 | Increased | 2.63E-01 | 2.63E-01 | NA | |
| Sputum | 3.50E-01 | 5.25E-01 | NA | 1.47E-02 | 4.42E-02 | NA | 1.04E-03 | 3.12E-03 | Increased | |
| Cluster C | ||||||||||
| NLF | Fractalkine, IL8, IP10, MIG | 1.85E-01 | 3.58E-01 | NA | 5.97E-01 | 5.97E-01 | NA | 4.83E-01 | 7.24E-01 | NA |
| NELF | 7.56E-01 | 9.43E-01 | NA | 2.62E-01 | 3.77E-01 | NA | 2.13E-01 | 2.63E-01 | NA | |
| Sputum | 2.80E-01 | 5.25E-01 | NA | 1.73E-01 | 2.60E-01 | NA | 3.89E-02 | 5.84E-02 | NA | |
Cytokines corresponding to each consensus cluster are detailed, as well as statistical results indicating instances of significant modulation associated with tobacco use at the cluster-level. NA, not applicable; NELF, nasal epithelial lining fluid; NLF, nasal lavage fluid.