Fig. 1. Sinapine directly inhibits oleic acid (OA)-induced TAG accumulation in HepG2 cell lines. The CCK-8 toxicity test of sinapine on HepG2 cells (A). OA-induced lipid accumulation in HepG2 cells stained with LipidTOX™ Green dye after 24 h (image, with 20× magnification, upper panel, (C)). Quantitative analyses were measured by the oil red O staining method and absorption was measured at 510 nm (B and C). Effects of different concentrations of sinapine on the expression of lipid metabolism genes in HepG2 cells (D). Each result represents the mean ± standard deviation (n = 10); different letters indicate significant differences (P < 0.05) between groups; the one-way ANOVA test followed by the Tukey test.