Primer sequences of genes used for quantitative real-time-polymerase chain reaction (qRT-PCR)a.
| Species | Gene | Forward | Reverse | Accession no. |
|---|---|---|---|---|
| Human | β-Actin | AAGGAGCCCCACGAGAAAAAT | ACCGAACTTGCATTGATTCCAG | NM_004882 |
| PPAR-α | GATGCCAGCGACTTTGACTC | ACCCACGTCATCTTCAGGGA | NM_001172698 | |
| ACOX2 | GCACCCCGACATAGAGAGC | CTGCGGAGTGCAGTGTTCT | NM_003500 | |
| SREBP-1c | TGCATTTTCTGACACGCTTC | CCAAGCTGTACAGGCTCTCC | NM_001005291.3 | |
| FAS | GTTGAGGGTCTGCGTGTCTTT | ACCCAGGGTGGCTAGAACA | NM_079422 | |
| Mouse | β-Actin | GCTACAGCTTCACCACCACA | AAGGAAGGCTGGAAAAGAGC | NM_027493 |
| PPAR-α | GCAGCTCGTACAGGTCATCA | ACTGCCGTTGTCTGTCACTG | NM_133947 | |
| ACOX1 | GTCTCGTCTTCGGCCTAGTG | TCGCTTGAGTGACCAGTGTC | NM_030721 | |
| SREBP-1c | CAGCTATTGGCCTTCCTCAG | GGTTACTGGCGGTCACTGTC | NM_011252.4 | |
| FAS | ATGCACACTCTGCGATGAAG | TTCAGGGTCATCCTGTCTCC | NM_019569 | |
| Dgat2 | TCGCGAGTACCTGATGTCTG | AGGGGGCGAAACCAATATAC | NM_026384 |
a
PPARα: peroxisome proliferator activated receptor α; ACOX2: acyl-CoA oxidase 2; ACOX1: acyl-CoA oxidase 1; SREBP-1c: sterol regulatory element-binding protein-1c; FAS: fatty acid synthase; Dgat2: diacylglycerol acyltransferase 2.