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. 2022 Apr 22;2022:9629158. doi: 10.1155/2022/9629158

Figure 4.

Figure 4

MiR-375-3p inhibitor alleviates the GPX4-related ferroptosis process in a cardiac fibroblast model (a). RNA of control fibroblasts, CF model cells, miRNA NC inhibitor treatment group, miR-375-3p inhibitor treatment group and Fer-1 treatment group were extracted, and miR-375-3p and mRNA levels of GPX4, COX2, ACSL4 and PTGS2 were detected by RT-PCR. (b). Western blotting was used to detect the protein expression of GPX4, COX2, ACSL4 and PTGS2 in cells of each treatment group. (c). Immunofluorescence was used to detect the protein levels of GPX4 (red signal) and ACSL4 (green signal) in cells of each treatment group. DAPI was used to display the nucleus morphology. Note.∗P < 0.05 vs. control, ∗∗P < 0.01 vs. control, #P < 0.01 vs. model and NC inhibitor, ##P < 0.01 model and NC inhibitor.