Figure 8.

EA exerts antiallodynic effect on CPIP rats through activating Nrf2 in affected hindpaw. (a) Nrf2 expression determined by Western blotting in ipsilateral hindpaw tissues of the control, CPIP, CPIP+EA, and CPIP+NAC groups 7 d after model establishment. The upper panel shows representative images of Nrf2, and the lower panel shows β-actin. (b) Summarized results of Nrf2 expression normalized to β-actin. Evaluation of SOD activity (c), GSH-Px activity (d), H₂O₂ (e), and MDA content (f) in ipsilateral hindpaw tissues from the CPIP+EA+ML385 and CPIP+EA+Veh group rats 7 d after model establishment. (g) Time course of the effect of intraplantar injecting Nrf2 antagonist ML385 (400 μg/rat) or corresponding vehicle (1% DMSO in PBS) on 50% PWT of ipsilateral hindpaws of CPIP model rats. (h) Summary of AUC as in (g). n = 6 rats/group. ^∗p < 0.05 and ^∗∗p < 0.01 vs. CPIP+EA+Veh or as indicated.