Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 10;27(2):819–830. doi: 10.1038/s41380-021-01182-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Expression of key neuronal channels in 1q21.1 deletion and duplication neurons following 50 days of neuronal differentiation. The values are presented as fold change compared to expression in controls. Data were analysed using multiple T tests (n ≥ 3) and significance is based on Holm–Sidak corrected P values. All data are presented as means ± SEM *P < 0.05; **P < 0.01; ***P < 0.001 ****P < 0.0001 vs. control. B Quantification of neuronal soma which show at least one characteristically neuronal calcium event in day 50 control and 1q21.1 deletion culture treated for 10 days with vehicle (DMSO) or verapamil (n ≥ 3/group). C Number of neuronal calcium events recorded per minute in day 50 control and 1q21.1 deletion cultures treated for 10 days with vehicle (DMSO) or verapamil. Both genotype (F_1,28 = 71.64; P < 0.0001; n ≥ 3/group) and the addition of verapamil (F_2,28 = 79.56; P < 0.0001; n ≥ 3/group) had significant effects on the average rate of calcium events. Furthermore, there was a significant interaction between the effect of genotype and drug (F_2,28 = 162.4; P < 0.0001; n ≥ 3/group) on the rate of calcium events. D Example traces of single neurons from both control and 1q21.1 deletion neurons treated with vehicle or verapamil. E Quantification of soma which show at least one characteristically neuronal calcium event in day 50 control and 1q21.1 deletion cultures treated for 10 days with vehicle (DMSO) or roscovitine. Only genotype had a significant effect on the percentage of active cells (F_1,22 = 463.9; P < 0.0001; n ≥ 3/group). F Number of characteristically neuronal calcium events recorded per minute in day 50 control and 1q21.1 duplication cultures treated for 10 days with vehicle (DMSO) or roscovitine. Both genotype (F_1,22 = 38.1; P < 0.0001; n ≥ 3/group) and the addition of roscovitine (F_2,22 = 63.87; P < 0.0001; n ≥ 3/group) had significant effects on the average rate of calcium events. Furthermore, there was a significant interaction between the effect of genotype and drug (F_2,22 = 16.06; P < 0.0001; n ≥ 3/group) on the rate of calcium events. G Representative traces of single neurons from both control and 1q21.1 duplication cultures treated with vehicle or roscovitine. Data sets were analysed by two-way ANOVA with post hoc comparisons using Dunnett’s multiple comparisons test comparing to control vehicle treated samples. Stars above points represent Dunnett-corrected post hoc tests. All data are presented as means ± SEM; ***P < 0.001 ****P < 0.0001 vs. vehicle treated control.