Fig. 4. Treatment with miR-20b and related oligomers produces parallel outcomes for APP mRNA, intracellular APP, and secreted APP.

HeLa cells were treated with oligomers: Specifically, miR-20b mimic, antagomiR to miR-20b, mimic + antagomiR, siRNA to APP, and a commercially obtained negative control mimic (NCM). Cultures and media were harvested and prepared for qRT-PCR, Western blotting, and LDH, as described in the text. Data were checked for multivariate normality then analyzed with MANOVA followed by estimated marginal means with the false discovery rate adjustment. Pillai’s trace (df, df): 2.17 (5, 1); p < 0.001, η² = 0.542. A Western blot of intracellular and secreted APP (sAPP), along with β-actin. B qRT-PCR relative outcomes, mock = 1. C Relative densitometry of intracellular APP, mock = 1. D Relative densitometry of sAPP, mock = 1. E Relative LDH signal, mock = 1. Symbols indicate statistical categories. Samples sharing a symbol do not differ at p ≤ 0.05. Error bars represent standard errors of means (SEM).