Skip to main content
. 2020 Jun 17;10(39):23173–23186. doi: 10.1039/d0ra03729j

Chronoamperometric detection modality-based biosensors.

Research Target, buffer Detection limit Experimental Reference
Disposable screen-printed amperometric biosensor utilizing monoclonal antibody as detection probe Progesterone, phenol solution, cattle milk samples 1 × 10−9 mol dm−3, 5 ng mL−1 +0.70 V vs. saturated calomel (SCE) 42 and 43
Immunosensor development using polypyrrole-based layer for entrapping bovine leukemia virus (BLV) protein using antibodies gp51 protein (BLV antigen), bovine serum 10-Fold dilutions of serum, antigen–antibody complex driven response, concentrations not mentioned 0 to 600 mV vs. Ag/AgCl, potential pulses of +950 mV vs. Ag/AgCl 44
Gold nanoparticle decorated polyaniline nanowire for covalent attachment of biorecognition elements – glucose oxidase enzyme (GOx), ssDNA, and lamin A antibody Glucose, complementary DNA strand, and lamin A protein, phosphate buffer solution 1 μM for glucose (chronoamperometric detection) Operating potentials of −0.1 V and +0.8 mV 45
Cellular biosensor for detection of aflatoxin B1 (AFB1) using screen-printed electrodes (SPEs) and anti AFB1 antibody as biorecognition element, and engineered/plain monkey vero kidney cells Aflatoxin B1 (AFB1)-mycotoxin, phosphate-buffered saline 0.05 ng mL−1 (plain cells) and 1.5 ng mL−1 (vero-anti-AFB1 cells) −100 mV potential 46
Chronoamperometric immunosensor using anti-TNF-α antibody functionalized on gold electrode using 4-carboxymethylaniline (CMA) Tumor necrosis factor (TNF) – α artificial saliva, saliva 1  pg mL−1 100 mV versus the saturated calomel reference electrode (SCE) 47