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. 2022 Apr 29;12:7045. doi: 10.1038/s41598-022-11015-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose and lactate levels in 24-well plate (24w) and BECA-D during EBVST culture. (A) Normalized glucose level (markers) in media collected and cell count (bar graph) in 24-well plate (24w) and BECA-D at the end of each activation. MC media chamber, CC cell chamber. Representative media change frequencies in 24-well plate and BECA-D are shown in Donor 3 graph. (B) Lactate concentration in media collected in 24-well plate (24w) and BECA-D at the end of each activation. (C) Plot of cell count against matched normalized glucose level in 24-well plate (24w) and BECA-D starting from 2nd activation, r value was calculated using Pearson correlation coefficient.