Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 29;13(4):417. doi: 10.1038/s41419-022-04862-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A ICAM-1 expression data in normal colon tissue (n = 54) and colorectal cancer tissue (n = 186) was obtained from the Gene Expression Omnibus (GEO) database. B Tissue microarray analysis of ICAM-1 expression in normal (n = 17) and cancer (n = 588) type of colorectal tissues. C qRT-PCR was performed to detect the expression of ICAM-1 in various colorectal cancer cell lines and normal colon cell. D, E Kaplan–Meier survival analysis cohorts based on low and high expression of ICAM-1 in colorectal cancer. (TCGA, n = 302). F GSEA (GSE4183) analysis was performed in colorectal cancer that ICAM-1 high expressed for hallmarks of cancer progression. NES normalized enrichment score; FDR q val false discovery rate q-value. G Transwell chamber assays were performed to determine whether expression of ICAM-1 modulates EMT after silencing ICAM-1 using SW-480 cells. H SW-480 cells transfected with sh-CTR and sh-ICAM-1 (3 × 10⁶) were injected into the cecal wall of athymic BALB/c-nude (8-weeks old) mice (n = 4). I Representative H&E staining images of lung metastases. And the graph shows the number of lung metastatic lesions in each mouse. J, K qRT-PCR and IHC staining of expression levels for EMT markers and regulators in tumor tissues of sh-CTR and sh-ICAM-1 injected mice. L Tube-forming ability of HUVEC cells was performed by incubation with SW-480 in which ICAM-1 was silenced. Tube formation was assessed after 2 h using light microscopy, and the Image J program was used to analyze tube length. M, N qRT-PCR and IHC staining of expression levels for that angiogenesis-related markers and growth factors in tumor tissues of sh-Ctr and sh-ICAM-1 injected mice. O Representative immunofluorescence image for CD31 (red) and VEGFA (green) in mouse tumors. Scale bar, 100 μm, β-actin and positive control were used as a control for normalization. Data are presented as mean ± SD and analyzed by Student’s t-tests. *P < 0.05; **P < 0.01; ***P < 0.001.