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. 2022 Apr 29;13(4):414. doi: 10.1038/s41419-022-04801-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–b LKB1 was immunoprecipitated from the HEK293T cells transduced with MDK-HA (a) and the HCCLM3 cells transduced with MDK shRNA1 (b), and then, Western blot analysis was performed with antibodies against STRADα, Mo25a, MDK and LKB1. c LKB1 was immunoprecipitated from the HEK293T cells transduced with MDK-HA and the control cells followed by Western blotting with antibodies against AMPKα, STRADα, Mo25a, HA, and FLAG. The indicated constructs were expressed in the HEK293T cells for 48 h, and the cell lysates were subjected to coimmunoprecipitation with primary LKB1 and IgG antibodies. d–e HEK293T cells were cotransfected with different doses of MDK-HA and SFB-tagged LKB1 (d) or SFB-tagged AMPKα1 (e), pulled down with S protein beads, and subjected to Western blot analysis with antibodies against STRADα, Mo25a, LKB1, MDK and AMPK subunits.