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. 2022 Apr 29;13(4):414. doi: 10.1038/s41419-022-04801-0

Fig. 6. Midkine promotes cancer cell proliferation, invasion and tumorigenesis.

Fig. 6

a–b Western blotting of MDK and β-actin in HCCLM3 cells transduced with two independent MDK shRNAs (a) and representative images and growth curves of the HCCLM3 cells with MDK knocked down (b). c–d Western blot analysis of MDK, Myc, HA, and β-actin in the MDK-transduced MHCC97H cells (c) and representative images and cell growth curves of MHCC97H cells overexpressing MDK (d). e–f Western blot analysis of MDK and β-actin in the HCCLM3 cells transduced with MDK shRNA1 and restored MDK in the MDK-knockdown cells (e) and representative images and cell growth curves of the HCCLM3 cells transduced with MDK shRNA1 and restored MDK in the MDK-knockdown cells (f). g Western blotting of MDK and HSP90 in the BT549 cells transduced with two independent MDK shRNAs and representative images and invaded cell numbers of BT549 cells with MDK knocked down. n = 3 wells per group. Scale bar, 200 μm. h Western blotting of MDK and β-actin in the MDK-overexpressing Bel-7402 cells and representative images and clone numbers of the Bel-7402 cells with restored MDK expression. n = 3 wells per group, Bar = 200 μm. i–k Tumor images (i), growth curve (j) and weight (k) after subcutaneously injecting mice with HCCLM3 cells transduced with MDK shRNA or reconstituted MDK in MDK-knockdown cells.