TABLE 1.

Growth phenotypes and MICs of mutants obtained via serial passage or chemical mutagenesis^a

Strain	Class	Growth phenotype on:				MIC (μg/ml)
		Liquid media (OD600)		Solid media (colony size)
		MHB	CDM	MHA	SCV	Dapto	Vanc	Amp	Nisin	Gent
Sa42	WT	6.05	6.6	+++	+++	0.78	0.78	6.25	3.125	0.78
Sa278b	1	5.93	7.87	+++	+++	12.5	1.56	6.25	25	0.78
Sa284	1	4.97	6.38	+++	+++	6.25	1.56	6.25	25	0.78
Sa285	2	1.23	NG	++	++	6.25	0.78	3.13	25	0.39
Sa286	3	0.63	NG	+	+	6.25	0.39	ND	6.25	0.78
Sa287	1	3.89	6.59	+++	+++	6.25	1.56	6.25	25	1.56
Sa288	1	5.34	8.17	+++	+++	6.25	0.78	ND	25	0.78
Sa289	3	0.69	0.45	+	+	12.5	0.39	ND	3.125	ND
Sa290	3	0.91	NG	+	+	12.5	0.39	ND	ND	1.56
Sa291	3	0.71	NG	+	+	25	0.39	1.56	3.125	0.78
Sa292	3	1.37	NG	+	+	12.5	0.78	ND	ND	0.78
Sa293	3	0.43	NG	+	+	12.5	0.78	ND	ND	1.56
Sa295	2	1.28	NG	++	++	12.5	1.56	6.25	6.25	<0.1

^aAbbreviations: OD₆₀₀, optical density at 600 nm; SCV, MHA plus hemin, thiamine, and menadione; Dapto, daptomycin; Vanc, vancomycin; Amp, ampicillin; Gent, gentamicin; WT, wild type; NG, no growth, 16 h; ND, not determined. 

^bObtained via serial passage techniques.