Fig. 5.

Loss of Notch4 altered embryonic dermal lymphangiogenesis. a–f Dermal lymphatic phenotype was determined for E14.5 Notch4^−/− (N4^−/−), Notch4^+/− (N4^+/−) and wild-type (WT) wholemounts stained for LYVE1. a Representative image of N4^−/− and N4^+/− dermal wholemounts. Red dotted line marks leading edge of lymphangiogenic front. Scale bars, 100 μm. b Quantification of the distance between migration fronts, normalized to the N4^+/− controls. Data presented ± s.e.m. One-way ANOVA: p = 0.014, t test: *p < 3 × 10⁻⁴, **p < 0.001. wt (n = 5), N4^+/− (n = 13), N4^−/− (n = 4). c Quantification of the number of lymphangiogenic sprouts normalized by length of the front. Data presented ± s.e.m. One-way ANOVA: p = 0.023, t test: *p < 0.02. WT (n = 6), N4^+/− (n = 11), N4^−/− (n = 6). d Quantification of the sprout length from the migration front to first branch-point. Data presented ± s.e.m. WT (n = 7), N4^+/− (n = 8), N4^−/− (n = 6). e Quantification of the average vessel caliber at the lymphangiogenic front and in the maturing plexus. Data presented ± s.e.m. One-way ANOVA: p = 0.019, t test: *p < 0.03, front analysis- WT (n = 7), N4^+/− (n=13), N4^−/− (n = 7), plexus analysis - WT (n = 6), N4^+/− (n = 9), N4^−/− (n = 8). f Representative image of the E14.5 maturing lymphatic plexus in N4^−/− and N4^+/− littermates. Scale bars, 100 μm. g–i Dermal lymphatic phenotype determined for E16.5 Notch4^−/− (N4^−/−) and wild-type (WT) wholemounts stained for VEGFR3. g Representative image of the E16.5 dermal lymphatic plexus in N4^−/− and WT littermates. Red arrowheads mark lymphatic valves. Scale bars, 100 μm; h quantification of mean distance between branch-points. Data presented ± s.e.m. t test: *p < 0.03 WT (n = 4), N4^−/− (n = 6). i Quantification of the average vessel caliber of the dermal lymphatic plexus at E16.5. Data presented ± s.e.m. WT (n = 6), N4^−/− (n = 8)