Fig. 8.

Loss of Notch4 and deletion of LEC canonical Notch signaling resulted in distinct lymphatic phenotypes at E17.5. a, b Lymphangiography of E17.5 wild-type (n = 6) and Notch4^−/− (n = 8) embryos. Representative images of wild-type and Notch4^−/− embryos. Boxed area enlarged to the right. b Notch4^−/− embryos with blood-filled dermal lymphatics (white arrowheads). Boxed area enlarged to the right. c, d Prox1CreER^T2 and DNMAML^fl/fl mice were crossed and tamoxifen administered at E12.5 and lymphangiography performed at E17.5. Control (n = 12), DNMAML^LEC (n = 5). c Representative images of DNMAML^fl/− control and DNMAML^LEC embryos. Boxed area enlarged to the right. d DNMAML^LEC embryo with leaking dermal lymphatic vessels (red arrowheads). Boxed area enlarged below