Table 1.

LC-gradients for methods A, B and C

	Method A			Method B			Method C
Step	Timea	Ab	Bc	Time	A	B	Time	A	B
1	0.25 min	100%	0%	0.25 min	100%	0%	0.25 min	100%	0%
2	6.60 min	20%	80%	6.60 min	0%	100%	4.00 min	60%	40%
3	13.00 min	0%	100%	12.00 min	0%	100%	6.60 min	0%	100%
4	13.01 min	100%	0%	12.01 min	100%	0%	9.01 min	100%	0%

^aTime indicates the time at which the indicated ratio of A/B is present

^bMobile phase A: 95% water + 5% acetonitrile + 0.01% formic acid + 5 mM ammonium formate

^cMobile phase B: 5% water + 95% acetonitrile + 0.01% formic acid + 5 mM ammonium formate