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. 2022 Apr 29;13:2361. doi: 10.1038/s41467-022-30084-2

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Fig. 2 — a DarA_pk produced by coexpression with DarE_pk carries a + 12 Da modification. Because heterologous expression in E. coli only produced obtained the truncated DarA_pk, the theoretically predicted signals corresponding to the unmodified DarA_pk were shown for comparison. See Supplementary Fig. 3 for the HR-MS/MS spectrum of DarA_pk(43-58)-1. b DarA_pk produced by coexpression with the C86A mutant of DarE_pk is a truncated peptide (i.e., DarA_pk(1-48)), which lacks the core and follower regions.