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. 2022 Apr 29;13:2361. doi: 10.1038/s41467-022-30084-2

Fig. 7. Production of a monocyclic daropeptide from a cluster IV BGC.

Fig. 7

a The BGC from P. asymbiotic, showing the genes and the precursor peptide sequence, and the core sequence is shown in bold font. Unlike other daropeptide BGCs, this BGC does not encode the DarBCD transporters but encodes a putative N-acetyltransferase. b The EIC at m/z = 770.36 ± 0.01 (corresponding to the monocyclic pentapeptide containing an ether crosslink) of DarApa-1 incubated with (i) the cell lysate of E. coli BL21 or (ii) the boiled cell lysate for 24 h. c HR-MS spectrum of the in vitro obtained monocyclic daropeptide. See Supplementary Figs. 17–19 for the data in characterization of the DarApa-derived peptides.