Fig. 5. MNPs-induced cellular responses involve concentration dependent remodeling of actin cytoskeleton. Phalloidin staining of the actin cytoskeleton in podocytes and mesangial cells after 24 h incubation with or without indicated concentrations of MNP-SO-PEG (A) or MNP-SO-BSA (B). Red – phalloidin, blue – DAPI. Magnification 630×. MNPs cause reduction of adhesive properties of podocytes and to a lesser extent of mesangial cells. Staining of focal adhesion molecule vinculin after 24 h incubation with or without 0.15 mM MNP-SO-PEG or MNP-SO-BSA (C). Red – phalloidin, green – vinculin, blue – DAPI. Magnification 630×.
