Figure 2.

Regulatory effects of six TFs on the CsPDX2.1 promoter and EMSA analysis of the binding ability of CsWRKY40 to the CsPDX2.1 promoter. (a) The Luc/Ren ratios of various TFs are all relative to the value of empty vector plus promoter, which was set to 1. Values are means (+SE) from three biological replicates (^****P < 0.0001). (b) The locations of the three W boxes in the promoter are shown, and the protein binding sequences and sites are highlighted. (c) Purified protein was mixed with three probes for EMSA. The presence (+) or absence (−) is shown. The biotin probe and mutant probe contained a 3′ biotin label, and the cold probe lacked the 3′ biotin label. The binding ability of the protein was analyzed by the concentration ratio of the cold probe to the biotin probe.