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The exosomes are secreted by H1299 cells. Exosomes were isolated from the culture medium of H1299 cells. (a) The exosomes were confirmed by TEM. Scale bar: 200 nm. (b) The diameter of exosomes was analyzed. (c) CD63 and CD81 levels were detected via Western blotting in the exosomes from the culture medium of H1299 cells with or without pre-treatment of 20 μM GW4869 for 2 h.
