Figure 7.

miR-1258 knockdown attenuates the influence of exosomal circ_0000519 downregulation on NSCLC cell growth and metastasis. H2170 and A549 cells were transfected with anti-NC or anti-miR-1258, and then, the transfected or non-transfected cells were incubated with the exosomes from the H1299 cells transfected with si-NC or si-circ_0000519. (a) miR-1258 expression was detected by qRT-PCR in the transfected cells. (b and c) Cell growth was evaluated via EdU staining and colony formation assays in the treated cells. (d and e) Cell migration and invasion were examined via transwell analysis in the treated cells. (f and g) Cyclin D1, Vimentin and MMP9 abundances were examined via Western blotting in the treated cells. ^*** P < 0.001.