Fig. 4. Optimizations of experimental parameters determined by Δf measurements of samples with various: (A) PLP hybridization temperatures, (B) concentrations of DNA ligase, (C) concentrations of CP-MB, (D) concentrations of Phi 29 DNA polymerase, (E) RCA reaction times, and (F) concentrations of nano probe. All concentrations are the initial reagent concentrations, and 1 pM of synthetic S. aureus DNA sequence was used in all optimization studies. Error bars indicate the standard deviation (n = 3 in all cases).