Figure 3.

Enzymatic cleavage of QLS-101 in vitro. (A) The ability of alkaline phosphatase (ALP) to cleave QLS-101 at physiological salt and pH was quantified at five minutes, 30 minutes, and two hours in a single experiment. (B, C) The ability of acid phosphatase (B) or 5ʹnucleotidase (C) to cleave QLS-101 at physiological salt and pH was quantified at five minutes, 30 minutes, and two hours in a single experiment. (D) Representative graph of three separate experiments quantifying ALP kinetics when incubated with QLS-101 in a non-phosphate physiological buffer (n = 2-3 per experiment). (E) Effect of pH on activity of ALP (21 nM) for QLS-101 was determined at pH 6.0 in MES buffer, as well as 7.4 and 9.0 in Tris buffer (n = 3). Data shown (D, E) are depicted as mean ± SD.