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| Gene | Species | Primer (5′–3′) | |
|---|---|---|---|
| ZO-1 | Rat | FORWARD | CCACCTCGCACGTATCACAAGC |
| REVERSE | GGCAATGACACTCCTTCGTCTCTG | ||
| Occludin | Rat | FORWARD | CCTCCTTACAGGCCGGATGA |
| REVERSE | AGCATTGGTCGAACGTGCAT | ||
| Claudin-1 | Rat | FORWARD | GTTTCATCCTGGCTTCGCTG |
| REVERSE | AGCAGTCACGATGTTGTCCC | ||
| RhoA | Rat | FORWARD | AGGCGGGAGTTAGCCAAAAT |
| REVERSE | GTACCCAAAAGCGCCAATCC | ||
| Rock | Rat | FORWARD | GGTGATGGAGTACATGCCAGGTG |
| REVERSE | ATCCAGTGCAAGCACGACTTCAG | ||
| GAPDH | Rat | FORWARD | CCCTCTGGAAAGCTGTGG |
| REVERSE | GCTTCACCACCTTCTTGATGT |
