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. Author manuscript; available in PMC: 2022 Sep 23.
Published in final edited form as: Methods Enzymol. 2021 Sep 23;659:37–70. doi: 10.1016/bs.mie.2021.07.007

Figure 2.

Figure 2.

Recombinant protein overexpression and characterization using IMAC-OBE-nMS. (A) General workflow and instrument setup. (B) Schematic of dual six-port switching valve system, which has two positions: “Load” (blue) and “Elute” (red). See Section 3.3.2 for more details. Adapted from (Busch et al., 2021) with permission from ACS Publications.