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. 2022 Apr 30;22:119. doi: 10.1186/s12906-022-03604-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Relative mRNA expression of IL-1β, IL-6 and IL-8 after TNFα pre-treatment of T24 and comparison of standard effects of eucalyptol and eucalyptus oils with ACHP treatment. The mRNA expression levels were determined with Real-time PCR using SYBR Green protocol. The relative expression of controls was regarded as 1. β-actin was utilized for normalization. A T24 cells were pre-treated with TNFα for 24 h then 6 h treated with ACHP/eucalyptol and eucalyptus oil. B T24 cells were pre-treated with TNFα for 6 h then 24 h treated with ACHP/ eucalyptol and eucalyptus oil (C) T24 cells were pre-treated with TNFα for 24 h then 24 h treated with ACHP/ eucalyptol and eucalyptus oil. DMSO treatment was used as a control of the treated cells. The experiments were repeated three times (n = 3) in triplicates. Asterisks indicate p < 0.05 compared to the control. Cross marks p < 0.05 compared to the ACHP treatment. Double cross shows p < 0.05 compared to the eucalyptol treatment