FIGURE 6.

Expression levels of Zbtb38 and markers in E7.5 embryos. Loss of Zbtb38 single allele results in a misregulation of pluripotency, proliferation, differentiation, and apoptotic genes in E7.5 embryos. (A) Schematic diagram of isolated E7.5 foetuses. (B and C) Confocal images of whole‐mount immunohistochemical staining (B) and relative fluorescent intensity (C). (B) Expressions of the indicated proteins were detected by anti‐Zbtb38, anti‐Nanog, and anti‐Sox2 antibodies. Cell nuclei were counterstained with DAPI. Scale bar denotes 50 μm. (C) Representative relative fluorescence as a measure of Zbtb38 and Nanog from the upper panel of (B) (fl/+: n = 2; ∆fl/+: n = 2), and a measure of Zbtb38 and Sox2 from the lower panel of (B) (fl/+: n = 2; ∆fl/+: n = 2). fl/+ was normalized to 1, and error bars indicate ± S.D. (D) qRT‐PCR results of the indicated gene expressions of the Zbtb38 ∆fl/+ embryo and control embryo. Data shown are representative of three independent experiments, and error bars represent ± S.D. *p < 0.05