FIGURE 1.

Upregulated insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) in granulosa cells (GCs) from polycystic ovary syndrome (PCOS) patients increases cell viability and proliferation. (A) IGF2BP2 mRNA detection by RT‐qPCR in GCs from Control and PCOS groups. Data were normalized to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH). Control: n = 20; PCOS: n = 20. *P < 0.05. (B) IGF2BP2 protein detection by western blot in GCs from PCOS and Control women. (C) RT‐qPCR showing expression of IGF2BP2 mRNA in IGF2BP2 overexpression (OE) KGN cells. ***P < 0.001. (D) IGF2BP2 protein detection by western blot in IGF2BP2 OE KGN cells. Experiments were performed in triplicate. (E) KGN cells were transfected with vector control or IGF2BP2 OE plasmids for 24, 48, or 72 h, and cell viability was determined by the CCK‐8 assay. ***P < 0.001. (F) EdU staining of IGF2BP2 OE cells. Nuclei were stained by using DAPI. EdU positive cells, red; cell nuclei, blue; the data shown were representative of three independent experiments with similar results. Scale bar, 100 μm. (G) Statistics of EdU positive cells quantified by counting the cells with fluorescent signal using the software Image J. Experiments were performed in triplicate. *P < 0.05. (H) Apoptosis was reflected by TUNEL staining of the cells from the indicated groups. Representative images of the apoptotic cells were shown. Scale bar, 100 μm. (I) Statistics of TUNEL positive cells quantified by counting the cells with fluorescent signal using the software Image J. *P < 0.05. Ctrl, control. OE, IGF2BP2 overexpression. Error bars represent the mean ± SEM