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. 2022 Mar 16;55(4):e13216. doi: 10.1111/cpr.13216

FIGURE 6.

FIGURE 6

Insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) overexpression (OE) regulates alternative splicing of nuclear factor 1 C‐type (NFIC). (A) IGF2BP2 binds to the mRNAs of NFIC. Genome visualization showing IGF2BP2‐regulated ASEs and relative binding on the left and right panels, respectively. The peak ranges were highlighted with purple or green colours. (B) Quantification of NFIC binding using RNA immunoprecipitation sequencing data. ***P < 0.001 (C) Schematic diagram of the NFIC AS (ENST00000341919.7). Boxes represent exons, lines between exons represent introns. (D) The altered ratios of ASEs in RNA‐seq were plotted. **P < 0.01. (E) RT‐qPCR validation of AS of NFIC in IGF2BP2 OE KGN cells. *P < 0.05. (F) The relative expression of CTF5 was detected using RT‐qPCR following IGF2BP2 OE in KGN cells. **P < 0.01. ASEs, alternative splicing events; ES, exon skipping. Error bars represent the mean ± SEM