FIGURE 7.

circ‐83756 regulated IVDD by modulating the miR‐558/TREM1 axis. (A) CCK8 assay detected the proliferation of NP cells co‐transfected with miR‐558 mimic and TREM1 OE. (B–E) Apoptosis rates were determined with flow cytometry and TUNEL assay. NP cells were co‐transfected with miR‐558 mimic and TREM1 OE. (F, G) The expressions of aggrecan, collagen Ⅱ, MMP3, ADAMTS5, IL‐6 and COX2 in NP cells were detected by WB. NP cells were co‐transfected with miR‐558 mimic and TREM1 OE. (H) The proliferation of NP cells co‐transfected with circ‐83756 OE and si‐TREM1 was detected by CCK8 assay. (I–L) Apoptosis rates were determined with flow cytometry and TUNEL assay. NP cells were co‐transfected with circ‐83756 OE and si‐TREM1. (M, N) The expressions of aggrecan, collagen Ⅱ, MMP3, ADAMTS5, IL‐6 and COX2 in NP cells were detected by WB. NP cells were co‐transfected with circ‐83756 OE and si‐TREM1. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the indicated group. Statistical data were presented as mean ±SEM; FITC, fluorescein isothiocyanate; PI, propidium iodide; TUNEL, terminal dexynucleotidyl transferase (TdT)‐mediated dUTP nick‐end labelling; DAPI, 4′,6‐diamidino‐2‐phenylindole