Fig. 5. (A) Determination of CMC points of copolymer via spectrofluorometric method (pyrene probe), where copolymer concentration: 0.01, 0.05, 0.5, 1, 2.5, 5, 10, 25, 50, 100, 250, 500, and 1000 μg mL⁻¹ in final solution, and pyrene concentration: 0.005 μg mL⁻¹ in final solution. (B) Drug release percentage versus time (day) plot, time intervals: 1, 2, 4, 6, 8, 10, 13, 15 and 17 days, 2 mg nano-formulation in 2 mL sink solution with pH = 7.4 and 5.5.