Sequences of the primers.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
CASPASE-3 | GAAATTGTGGAATTGATGCGTGA | CTACAACGATCCCCTCTGAAAAA |
CASPASE-6 | ATGGCGAAGGCAATCACATTT | GTGCTGGTTTCCCCGACAT |
CASPASE-7 | AGGGACCGAGCTTGATGATG | CACTGGGATCTTGTATCGAGGA |
CASPASE-8 | GATCAAGCCCCACGATGAC | CCTGTCCATCAGTGCCATAG |
CASPASE-9 | CTTCGTTTCTGCGAACTAACAGG | GCACCACTGGGGTAAGGTTT |
CASPASE-10 | AGAAACCTGCTCTACGAACTGT | GGGAAGCGAGTCTTTCAGAAG |
CASPASE-12 | TGTTACAAAGGCTCATGTGGAAA | GGGTCAGTATATTTGGGGTCTCA |
Bax | TTCTGACGGCAACTTCAACT | CAGCCCATGATGGTTCTGAT |
Bcl-2 | GGGAATCGATCTGGAAATCCTC | GGCAACGATCCCATCAATCT |
GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |